GPCRDB: Swiss-Prot entry

ID   GNAI2_RAT      STANDARD;      PRT;   354 AA.
AC   P04897;
DT   13-AUG-1987 (Rel. 05, Created)
DT   01-OCT-1994 (Rel. 30, Last sequence update)
DT   10-MAY-2005 (Rel. 47, Last annotation update)
DE   Guanine nucleotide-binding protein G(i), alpha-2 subunit (Adenylate
DE   cyclase-inhibiting G alpha protein).
GN   Name=Gnai2; Synonyms=Gnai-2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; 
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; 
OC   Muridae; Murinae; Rattus. 
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=86233317; PubMed=3086867 [NCBI, ExPASy, EBI, Israel, Japan];
RA   Itoh H., Kozasa T., Nagata S., Nakamura S., Katada T., Ui M., Iwai S.,
RA   Ohtsuka E., Kawasaki H., Suzuki K., Kaziro Y.;
RT   "Molecular cloning and sequence determination of cDNAs for alpha
RT   subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go
RT   from rat brain.";
RL   Proc. Natl. Acad. Sci. U.S.A. 83:3776-3780(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=88007678; PubMed=2820999 [NCBI, ExPASy, EBI, Israel, Japan];
RA   Jones D.T., Reed R.R.;
RT   "Molecular cloning of five GTP-binding protein cDNA species from rat
RT   olfactory neuroepithelium.";
RL   J. Biol. Chem. 262:14241-14249(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   PROTEIN SEQUENCE OF 11-125.
RX   PubMed=2159473 [NCBI, ExPASy, EBI, Israel, Japan];
RA   Linder M.E., Ewald D.A., Miller R.J., Gilman A.G.;
RT   "Purification and characterization of Go alpha and three types of Gi
RT   alpha after expression in Escherichia coli.";
RL   J. Biol. Chem. 265:8243-8251(1990).
CC   -!- FUNCTION: Guanine nucleotide-binding proteins (G proteins) are
CC       involved as modulators or transducers in various transmembrane
CC       signaling systems. The G(i) proteins are involved in hormonal
CC       regulation of adenylate cyclase: they inhibit the cyclase in
CC       response to beta-adrenergic stimuli.
CC   -!- SUBUNIT: G proteins are composed of 3 units; alpha, beta and
CC       gamma. The alpha chain contains the guanine nucleotide binding
CC       site. Interacts with UNC5B (By similarity).
CC   -!- SIMILARITY: Belongs to the G-alpha family. G(i/o/t/z) subfamily.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; M12672; AAA41260.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR   EMBL; M17528; AAA40824.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR   EMBL; BC078806; AAH78806.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR   PIR; D27423; RGRTI2.
DR   HSSP; P10824; 1AS3. [HSSP ENTRY / SWISS-3DIMAGE / PDB]
DR   SMR; P04897; 4-353.
DR   Ensembl; ENSRNOG00000016592; Rattus_norvegicus
DR   RGD; 620243; Gnai2.
DR   GO; GO:0007243; P:protein kinase cascade; IMP.
DR   InterPro; IPR001019; Gprotein_alpha.
DR   InterPro; IPR001408; Gprotein_alpha_I.
DR   InterPro; IPR011025; GproteinA_insert.
DR   InterPro; Graphical view of domain structure.
DR   Pfam; PF00503; G-alpha; 1.
DR   Pfam; Graphical view of domain structure.
DR   PRINTS; PR00318; GPROTEINA.
DR   PRINTS; PR00441; GPROTEINAI.
DR   ProDom; PD000281; Gprotein_alpha; 1.
DR   ProDom [Domain structure / List of seq. sharing at least 1 domain ]
DR   CMR; P04897.
DR   HOVERGEN [Family / Alignment / Tree]
DR   BLOCKS; P04897.
DR   ProtoNet; P04897.
DR   ProtoMap; P04897.
DR   PRESAGE; P04897.
DR   DIP; P04897.
DR   ModBase; P04897.
DR   SWISS-2DPAGE; GET REGION ON 2D PAGE.
KW   ADP-ribosylation; Direct protein sequencing; GTP-binding; Lipoprotein;
KW   Multigene family; Myristate; Palmitate; Transducer.
FT   INIT_MET      0      0       By similarity.
FT   NP_BIND      39     46       GTP (By similarity).
FT   NP_BIND     200    204       GTP (By similarity).
FT   NP_BIND     269    272       GTP (By similarity).
FT   MOD_RES     178    178       ADP-ribosylarginine (by cholera toxin).
FT   MOD_RES     351    351       ADP-ribosylcysteine (by pertussis toxin).
FT   LIPID         1      1       N-myristoyl glycine (By similarity).
FT   LIPID         2      2       S-palmitoyl cysteine (By similarity).
FT   VARIANT     165    166       SD -> PN (in tryptic peptides).
SQ   SEQUENCE   354 AA;  40368 MW;  436B75599113FC19 CRC64;
     GCTVSAEDKA AAERSKMIDK NLREDGEKAA REVKLLLLGA GESGKSTIVK QMKIIHEDGY
     SEEECRQYRA VVYSNTIQSI MAIVKAMGNL QIDFADPQRA DDARQLFALS CAAEEQGMLP
     EDLSGVIRRL WADHGVQACF GRSREYQLND SAAYYLNDLE RIAQSDYIPT QQDVLRTRVK
     TTGIVETHFT FKDLHFKMFD VGGQRSERKK WIHCFEGVTA IIFCVALSAY DLVLAEDEEM
     NRMHESMKLF DSICNNKWFT DTSIILFLNK KDLFEEKITQ SPLTICFPEY TGANKYDEAA
     SYIQSKFEDL NKRKDTKEIY THFTCATDTK NVQFVFDAVT DVIIKNNLKD CGLF
//