GPCRDB: Swiss-Prot entry
ID GNAI2_ORYLA STANDARD; PRT; 354 AA.
AC O13055;
DT 28-FEB-2003 (Rel. 41, Created)
DT 28-FEB-2003 (Rel. 41, Last sequence update)
DT 10-MAY-2005 (Rel. 47, Last annotation update)
DE Guanine nucleotide-binding protein G(i), alpha-2 subunit (Adenylate
DE cyclase-inhibiting G alpha protein) (Gi2 alpha subunit) (Gi alpha c).
GN Name=GNAI2;
OS Oryzias latipes (Medaka fish) (Japanese ricefish).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Euteleostei; Neoteleostei;
OC Acanthomorpha; Acanthopterygii; Percomorpha; Atherinomorpha;
OC Beloniformes; Adrianichthyidae; Oryziinae; Oryzias.
OX NCBI_TaxID=8090;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Ovary;
RX MEDLINE=98055713; PubMed=9395335 [NCBI, ExPASy, EBI, Israel, Japan];
RA Oba Y., Yoshikuni M., Tanaka M., Mita M., Nagahama Y.;
RT "Inhibitory guanine-nucleotide-binding-regulatory protein alpha
RT subunits in medaka (Oryzias latipes) oocytes -- cDNA cloning and
RT decreased expression of proteins during oocyte maturation.";
RL Eur. J. Biochem. 249:846-853(1997).
CC -!- FUNCTION: Guanine nucleotide-binding proteins (G proteins) are
CC involved as modulators or transducers in various transmembrane
CC signaling systems. The G(i) proteins are involved in hormonal
CC regulation of adenylate cyclase: they inhibit the cyclase in
CC response to beta-adrenergic stimuli.
CC -!- SUBUNIT: G proteins are composed of 3 units; alpha, beta and
CC gamma. The alpha chain contains the guanine nucleotide binding
CC site.
CC -!- SIMILARITY: Belongs to the G-alpha family. G(i/o/t/z) subfamily.
CC --------------------------------------------------------------------------
CC This Swiss-Prot entry is copyright. It is produced through a collaboration
CC between the Swiss Institute of Bioinformatics and the EMBL outstation -
CC the European Bioinformatics Institute. There are no restrictions on its
CC use as long as its content is in no way modified and this statement is not
CC removed.
CC --------------------------------------------------------------------------
DR EMBL; AB001742; BAA20073.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR HSSP; P10824; 1GDD. [HSSP ENTRY / SWISS-3DIMAGE / PDB]
DR SMR; O13055; 4-353.
DR InterPro; IPR001019; Gprotein_alpha.
DR InterPro; IPR001408; Gprotein_alpha_I.
DR InterPro; IPR011025; GproteinA_insert.
DR InterPro; Graphical view of domain structure.
DR Pfam; PF00503; G-alpha; 1.
DR Pfam; Graphical view of domain structure.
DR PRINTS; PR00318; GPROTEINA.
DR PRINTS; PR00441; GPROTEINAI.
DR ProDom; PD000281; Gprotein_alpha; 1.
DR ProDom [Domain structure / List of seq. sharing at least 1 domain ]
DR SMART; SM00275; G_alpha; 1.
DR HOVERGEN [Family / Alignment / Tree]
DR BLOCKS; O13055.
DR ProtoNet; O13055.
DR ProtoMap; O13055.
DR PRESAGE; O13055.
DR DIP; O13055.
DR ModBase; O13055.
DR SWISS-2DPAGE; GET REGION ON 2D PAGE.
KW ADP-ribosylation; GTP-binding; Lipoprotein; Multigene family;
KW Myristate; Palmitate; Transducer.
FT INIT_MET 0 0 By similarity.
FT NP_BIND 39 46 GTP (By similarity).
FT NP_BIND 200 204 GTP (By similarity).
FT NP_BIND 269 272 GTP (By similarity).
FT MOD_RES 178 178 ADP-ribosylarginine (by cholera toxin)
FT (By similarity).
FT MOD_RES 351 351 ADP-ribosylcysteine (by pertussis toxin)
FT (By similarity).
FT LIPID 1 1 N-myristoyl glycine (By similarity).
FT LIPID 2 2 S-palmitoyl cysteine (By similarity).
SQ SEQUENCE 354 AA; 40862 MW; C5D64B0970E3BDD3 CRC64;
GCTVSQEDKA AADRSKMIDK NLREDGEKAA REVKLLLLGA GESGKSTIVK QMKIIHEDGY
SEDECKQYRA VVYSNTVQSI MAIIKAMTIL HLDYERPERE EDAKRLFKMA AEAEERGDLP
EELANIIKDL WADSGIQHCL TRAREYQLND SAAYYLKDLE RISKPDYIPT QQDVLRTRVK
TTGIVETHFT FKDLHFKMFD VGGQRSERKK WIHCFEGVTA IIFCVAMSAY DLVLAEDEEM
NRMHESMKLF DSICNNKWFT ETSIILFLNK KDLFEQKIAH SPLTICFPEY EGPNTYQEAQ
AYIQTKFEDL NKKKETKEIY THFTCATDTK NVQFVFDAVT DVIIKNNLKD CGLF
//