GPCRDB: Swiss-Prot entry
ID GNAI1_XENLA STANDARD; PRT; 353 AA.
AC P27044;
DT 01-AUG-1992 (Rel. 23, Created)
DT 01-OCT-1994 (Rel. 30, Last sequence update)
DT 10-MAY-2005 (Rel. 47, Last annotation update)
DE Guanine nucleotide-binding protein G(i), alpha-1 subunit (Adenylate
DE cyclase-inhibiting G alpha protein).
GN Name=GNAI1;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Amphibia; Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae;
OC Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Oocyte;
RX MEDLINE=90346157; PubMed=2116977 [NCBI, ExPASy, EBI, Israel, Japan]; DOI=10.1016/0014-5793(90)80964-K;
RA Olate J., Martinez S., Purcell P., Jorquera H., Codina J.,
RA Birnbaumer L., Allende J.E.;
RT "Molecular cloning and sequence determination of four different cDNA
RT species coding for alpha-subunits of G proteins from Xenopus laevis
RT oocytes.";
RL FEBS Lett. 268:27-31(1990).
CC -!- FUNCTION: Guanine nucleotide-binding proteins (G proteins) are
CC involved as modulators or transducers in various transmembrane
CC signaling systems. The G(i) proteins are involved in hormonal
CC regulation of adenylate cyclase: they inhibit the cyclase in
CC response to beta-adrenergic stimuli.
CC -!- SUBUNIT: G proteins are composed of 3 units; alpha, beta and
CC gamma. The alpha chain contains the guanine nucleotide binding
CC site.
CC -!- SIMILARITY: Belongs to the G-alpha family. G(i/o/t/z) subfamily.
CC --------------------------------------------------------------------------
CC This Swiss-Prot entry is copyright. It is produced through a collaboration
CC between the Swiss Institute of Bioinformatics and the EMBL outstation -
CC the European Bioinformatics Institute. There are no restrictions on its
CC use as long as its content is in no way modified and this statement is not
CC removed.
CC --------------------------------------------------------------------------
DR EMBL; X56089; CAA39569.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR PIR; S11045; RGXLI1.
DR HSSP; P10824; 1AS3. [HSSP ENTRY / SWISS-3DIMAGE / PDB]
DR SMR; P27044; 4-347.
DR InterPro; IPR001019; Gprotein_alpha.
DR InterPro; IPR001408; Gprotein_alpha_I.
DR InterPro; IPR011025; GproteinA_insert.
DR InterPro; Graphical view of domain structure.
DR Pfam; PF00503; G-alpha; 1.
DR Pfam; Graphical view of domain structure.
DR PRINTS; PR00318; GPROTEINA.
DR PRINTS; PR00441; GPROTEINAI.
DR ProDom; PD000281; Gprotein_alpha; 1.
DR ProDom [Domain structure / List of seq. sharing at least 1 domain ]
DR HOVERGEN [Family / Alignment / Tree]
DR BLOCKS; P27044.
DR ProtoNet; P27044.
DR ProtoMap; P27044.
DR PRESAGE; P27044.
DR DIP; P27044.
DR ModBase; P27044.
DR SWISS-2DPAGE; GET REGION ON 2D PAGE.
KW ADP-ribosylation; GTP-binding; Lipoprotein; Multigene family;
KW Myristate; Palmitate; Transducer.
FT INIT_MET 0 0 By similarity.
FT NP_BIND 39 46 GTP (By similarity).
FT NP_BIND 199 203 GTP (By similarity).
FT NP_BIND 268 271 GTP (By similarity).
FT MOD_RES 177 177 ADP-ribosylarginine (by cholera toxin).
FT MOD_RES 350 350 ADP-ribosylcysteine (by pertussis toxin).
FT LIPID 1 1 N-myristoyl glycine (By similarity).
FT LIPID 2 2 S-palmitoyl cysteine (By similarity).
SQ SEQUENCE 353 AA; 40270 MW; 6B4EE94F841B077D CRC64;
GCTLSAEDKA AVERSKMIDR NLREDGEKAA REVKLLLLGA GESGKSTIVK QMKIIHEAGY
SEEECKQYKA VVYSNTIQSI IAIIRAMGRL KIDFGDPSRA DDARQLFVLA GAAEEGFMTA
ELAGVIKRLW KDGGVQACFN RSREYQLNDS AAYYLNDLDR IAQNSYIPTQ QDVLRTRVKT
TGIVETHFTF KDLHFKMFDV GGQRSERKKW IHCFEGVTAI IFCVALSDYD LVLAEDEEMN
RMHESMKLFD SICNNKWFTD TSIILFLNKK DLFEEKIKRS PLTICYPEYP GSNTYEEAAA
YIQCQFEDLN KRKDTKEIYT HFTCATDTKN VQFVFDAVTD VIIKNNLKDC GLF
//