Predict the effect of a point-mutation
Predict the effect of a point mutant
This service can predict the effects of a point mutation. You
will be taken through a number of steps to select the protein you want
to mutate, select the mutated residue and the residue type of the mutant.
Detailed help is available for all steps.
Theory
For more detailed information about how this service works its magic, please look here.
Predict the effect of a point mutant
This service can predict the effects of a point mutation. You
will be taken through a number of steps to select the protein you want
to mutate, select the mutated residue and the residue type of the mutant.
Detailed help is available for all steps.
Theory
For more detailed information about how this service works its magic, please look here.
Step 1: Select the protein to mutate
Protein search help
This search form contains a number of fields, all of which are
optional (although at least one field is needed to perform a search).
The search terms you enter are 'ANDed' together, meaning that the
proteins that are found comply with ALL the terms you have specified.
Id's and accession codes
The identifiers in this database correspond to the ID fields in
UniProt records and records from other databases. These look like
'adrb2_human' and 'opsd_bovin'. To search for entries like 'P07550' use
the accession code field.
Gene names
A large number of proteins is linked to a gene, for which you
can search using the 'gene' box. Searching is case insensitive.
Descriptions
Most proteins have a short description of their
function. These descriptions can be searched by using the description
field. Searching is case insensitive and wildcards are used on both
sides of your query, so 'rhodop' and 'opsin' will both match a
description containing the word 'Rhodopsin'.
Species
You can search for proteins from certain species by entering a
(part of) the scientific name of the species. If you would like to
search for human proteins, entering 'homo sapiens' in the species
search field would do just that.
Families
Using the families search box you can search for proteins that
have a specific family name. This search is done both on all the
subfamily levels: searching for 'amine' will return all proteins in the
amine protein family (as well as proteins from protein families which
have amine in their family name). Searching is case insensitive and
wildcards are used on both sides of your query, so 'adrenoceptor' will
match all the beta-adrenoceptor and alpha-adrenoceptor families
Mutant data
Selecting the option 'has mutant data' will return only proteins
for which there is mutant data available.
Structure data
Selecting the option 'has structure data' will return only
proteins for which there is structure data available.
Oligomer data
Selecting the option 'has oligomer data' will return only proteins
for which there is oligomerization data available.
Ligand binding data
Selecting the option 'has ligand data' will return only proteins
for which there is ligand binding data available.
Protein search help
This search form contains a number of fields, all of which are optional (although at least one field is needed to perform a search). The search terms you enter are 'ANDed' together, meaning that the proteins that are found comply with ALL the terms you have specified.
Id's and accession codes
The identifiers in this database correspond to the ID fields in UniProt records and records from other databases. These look like 'adrb2_human' and 'opsd_bovin'. To search for entries like 'P07550' use the accession code field.
Gene names
A large number of proteins is linked to a gene, for which you can search using the 'gene' box. Searching is case insensitive.
Descriptions
Most proteins have a short description of their function. These descriptions can be searched by using the description field. Searching is case insensitive and wildcards are used on both sides of your query, so 'rhodop' and 'opsin' will both match a description containing the word 'Rhodopsin'.
Species
You can search for proteins from certain species by entering a (part of) the scientific name of the species. If you would like to search for human proteins, entering 'homo sapiens' in the species search field would do just that.
Families
Using the families search box you can search for proteins that have a specific family name. This search is done both on all the subfamily levels: searching for 'amine' will return all proteins in the amine protein family (as well as proteins from protein families which have amine in their family name). Searching is case insensitive and wildcards are used on both sides of your query, so 'adrenoceptor' will match all the beta-adrenoceptor and alpha-adrenoceptor families
Mutant data
Selecting the option 'has mutant data' will return only proteins for which there is mutant data available.
Structure data
Selecting the option 'has structure data' will return only proteins for which there is structure data available.
Oligomer data
Selecting the option 'has oligomer data' will return only proteins for which there is oligomerization data available.
Ligand binding data
Selecting the option 'has ligand data' will return only proteins for which there is ligand binding data available.
Select the protein you would like to mutate by searching the database and selecting your protein.
To search, specify a (part of a) protein identifier and click the 'Search' button.